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Title Operation of a UV-Vis Spectrophotometer
Description Demonstrates the general procedures used to prepare a reference and samples for UV-Vis spectroscopic analysis.

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Subsections
1 Diode-Array Spectrophotometer
2 Marking the Cuvette
3 Preparing the Reference
4 Measuring the Reference
5 Measuring the Sample
6 Analyzing Multiple Samples
Concepts concentration, absorbance, transmittance, standards, Beer's Law, UV-Vis spectroscopy, reference, sample
Equipment Beaker
Kimwipes®
Spectrophotometer
Cuvette
Pen
Chemicals Copper(II) sulfate, CuSO4
Deionized water, H2O